{"id":9941,"date":"2022-06-24T17:38:04","date_gmt":"2022-06-24T17:38:04","guid":{"rendered":"http:\/\/www.sci.muni.cz\/ofiz\/?p=9941"},"modified":"2022-12-01T17:38:54","modified_gmt":"2022-12-01T17:38:54","slug":"binding-of-dep-domain-to-phospholipid-membranes-more-than-just-electrostatics","status":"publish","type":"post","link":"https:\/\/ueb1.sci.muni.cz\/ofiz\/binding-of-dep-domain-to-phospholipid-membranes-more-than-just-electrostatics\/","title":{"rendered":"Binding of DEP domain to phospholipid membranes: More than just electrostatics"},"content":{"rendered":"\n<p><strong>Abstract:<\/strong><\/p>\n\n\n\n<p>Over the past decades an extensive effort has been made to\nprovide a more comprehensive understanding of Wnt signaling, yet many\nregulatory and structural aspects remain elusive. Among these, the ability of\nDishevelled (DVL) protein to relocalize at the plasma membrane is a crucial\nstep in the activation of all Wnt pathways. The membrane binding of DVL was\nsuggested to be mediated by the preferential interaction of its C-terminal DEP\ndomain with phosphatidic acid (PA). However, due to the scarcity and fast\nturnover of PA, we investigated the role on the membrane association of other\nmore abundant phospholipids. The combined results from computational\nsimulations and experimental measurements with various model phospholipid\nmembranes, demonstrate that the membrane binding of DEP\/DVL constructs is\ngoverned by the concerted action of generic electrostatics and finely-tuned\nintermolecular interactions with individual lipid species. In particular, while\nwe confirmed the strong preference for PA lipid, we also observed a weak but\nnon-negligible affinity for phosphatidylserine, the most abundant anionic\nphospholipid in the plasma membrane, and phosphatidylinositol 4,5-bisphosphate.\nThe obtained molecular insight into DEP-membrane interaction helps to elucidate\nthe relation between changes in the local membrane composition and the\nspatiotemporal localization of DVL and, possibly, other DEP-containing\nproteins.<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"538\" src=\"https:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/depdomain-1024x538.jpg\" alt=\"\" class=\"wp-image-9942\" srcset=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/depdomain-1024x538.jpg 1024w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/depdomain-300x158.jpg 300w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/depdomain-768x403.jpg 768w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/depdomain-740x388.jpg 740w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/depdomain-370x194.jpg 370w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/depdomain-848x445.jpg 848w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/depdomain-1120x588.jpg 1120w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/depdomain-560x294.jpg 560w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/depdomain.jpg 1577w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p><strong>Authors:<\/strong><\/p>\n<p>Francesco L Falginella 1, <strong>Marek Kravec 2<\/strong>, Martina Drabinov\u00e1 3, <strong>Petra Pacl\u00edkov\u00e1 2<\/strong>, <strong>V\u00edt\u0115zslav Bryja 2 4<\/strong>, Robert V\u00e1cha 5<\/p>\n<p><strong>Affiliations:<\/strong><\/p>\n<p>1 CEITEC &#8211; Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.<\/p>\n<p><strong>2 Department of Experimental Biology, Faculty of Science, Masaryk University, Brno 62500, Czech Republic.<\/strong><\/p>\n<p>3 CEITEC &#8211; Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.<\/p>\n<p>4 Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Brno 612 65, Czech Republic.<\/p>\n<p>5 CEITEC &#8211; Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic; Department of Condensed Matter Physics, Faculty of Science, Masaryk University, Kotl\u00e1\u0159sk\u00e1 2, 611 37 Brno, Czech Republic.<\/p>\n\n\n<style>.wp-block-kadence-advancedbtn.kb-btns_9c45ee-43{gap:var(--global-kb-gap-xs, 0.5rem );justify-content:center;align-items:center;}.kt-btns_9c45ee-43 .kt-button{font-weight:normal;font-style:normal;}.kt-btns_9c45ee-43 .kt-btn-wrap-0{margin-right:5px;}.wp-block-kadence-advancedbtn.kt-btns_9c45ee-43 .kt-btn-wrap-0 .kt-button{color:#fff;background:#009900;border-color:#fff;box-shadow:1px 1px 2px 3px rgba(0, 0, 0, 0.2);}.wp-block-kadence-advancedbtn.kt-btns_9c45ee-43 .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns_9c45ee-43 .kt-btn-wrap-0 .kt-button:focus{color:#ffffff;border-color:#444444;}.wp-block-kadence-advancedbtn.kt-btns_9c45ee-43 .kt-btn-wrap-0 .kt-button::before{display:none;}.wp-block-kadence-advancedbtn.kt-btns_9c45ee-43 .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns_9c45ee-43 .kt-btn-wrap-0 .kt-button:focus{background:#444444;}<\/style>\n<div class=\"wp-block-kadence-advancedbtn kt-btn-align-center kt-btn-tablet-align-inherit kt-btn-mobile-align-inherit kt-btns-wrap kt-btns_9c45ee-43\"><div class=\"kt-btn-wrap kt-btn-wrap-0\"><a class=\"kt-button kt-btn-0-action kt-btn-size-standard kt-btn-style-basic kt-btn-svg-show-always kt-btn-has-text-true kt-btn-has-svg-false\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35750206\/\"><span class=\"kt-btn-inner-text\">Read the whole story<\/span><\/a><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Abstract: Over the past decades an extensive effort has been made to provide a more comprehensive understanding of Wnt signaling, yet many regulatory and structural aspects remain elusive. Among these, the ability of Dishevelled (DVL) protein to relocalize at the &hellip; <a href=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/binding-of-dep-domain-to-phospholipid-membranes-more-than-just-electrostatics\/\">Read More<\/a><\/p>\n","protected":false},"author":5,"featured_media":9942,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"kt_blocks_editor_width":"","_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[412,252],"tags":[304],"class_list":["post-9941","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bryjalab","category-nova-publikace","tag-bryjalab"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.1.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Binding of DEP domain to phospholipid membranes: More than just electrostatics - Department of Animal Physiology and Immunology<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/binding-of-dep-domain-to-phospholipid-membranes-more-than-just-electrostatics\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Binding of DEP domain to phospholipid membranes: More than just electrostatics - Department of Animal Physiology and Immunology\" \/>\n<meta property=\"og:description\" content=\"Abstract: Over the past decades an extensive effort has been made to provide a more comprehensive understanding of Wnt signaling, yet many regulatory and structural aspects remain elusive. 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