{"id":9936,"date":"2022-04-25T17:14:43","date_gmt":"2022-04-25T17:14:43","guid":{"rendered":"http:\/\/www.sci.muni.cz\/ofiz\/?p=9936"},"modified":"2022-12-01T17:15:52","modified_gmt":"2022-12-01T17:15:52","slug":"luminocell-a-versatile-and-affordable-platform-for-real-time-monitoring-of-luciferase-based-reporters","status":"publish","type":"post","link":"https:\/\/ueb1.sci.muni.cz\/ofiz\/luminocell-a-versatile-and-affordable-platform-for-real-time-monitoring-of-luciferase-based-reporters\/","title":{"rendered":"LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters"},"content":{"rendered":"\n<p><strong>Abstract:<\/strong><\/p>\n<p>Luciferase reporter assays represent a simple and sensitive experimental system in cell and molecular biology to study multiple biological processes. However, the application of these assays is often limited by the costs of conventional luminometer instruments and the versatility of their use in different experimental conditions. Therefore, we aimed to develop a small, affordable luminometer allowing continuous measurement of luciferase activity, designed for inclusion into various kinds of tissue culture incubators. Here, we introduce LuminoCell-an open-source platform for the construction of an affordable, sensitive, and portable luminometer capable of real-time monitoring in-cell luciferase activity. The LuminoCell costs $40, requires less than 1 h to assemble, and it is capable of performing real-time sensitive detection of both magnitude and duration of the activity of major signalling pathways in cell cultures, including receptor tyrosine kinases (EGF and FGF), WNT\/\u03b2-catenin, and NF-\u03baB. In addition, we show that the LuminoCell is suitable to be used in cytotoxicity assays as well as for monitoring periodic circadian gene expression.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"679\" height=\"841\" src=\"https:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/luminocell.jpg\" alt=\"\" class=\"wp-image-9937\" srcset=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/luminocell.jpg 679w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/luminocell-242x300.jpg 242w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/luminocell-560x694.jpg 560w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/luminocell-370x458.jpg 370w\" sizes=\"auto, (max-width: 679px) 100vw, 679px\" \/><\/figure><\/div>\n\n\n\n<p><strong>Authors:<\/strong><\/p>\n<p>Kamila Weissov\u00e1 1, Bohumil Faf\u00edlek 2 3 4, <strong>Tomasz Radaszkiewicz 5<\/strong>, Canan Celiker 1, Petra Mach\u00e1\u010dkov\u00e1 6, Tamara \u010cechov\u00e1 3 4, <strong>Jana \u0160ebest\u00edkov\u00e1 1 5<\/strong>, Ale\u0161 Hampl 1 4, <strong>V\u00edt\u011bzslav Bryja 5<\/strong>, Pavel Krej\u010d\u00ed 2 3 4, <strong>Tom\u00e1\u0161 B\u00e1rta 7 5<\/strong><\/p>\n<p><strong>Affiliations:<\/strong><\/p>\n<p>1 Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.<\/p>\n<p>2 Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Brno, Czech Republic.<\/p>\n<p>3 Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.<\/p>\n<p>4 International Clinical Research Center, St. Anne&#8217;s University Hospital, Brno, Czech Republic.<\/p>\n<p><strong>5 Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.<\/strong><\/p>\n<p>6 Cellular Imaging Core Facility, Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic.<\/p>\n<p>7 Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic tbarta@med.muni.cz.<\/p>\n\n\n<style>.wp-block-kadence-advancedbtn.kb-btns_a301a2-55{gap:var(--global-kb-gap-xs, 0.5rem );justify-content:center;align-items:center;}.kt-btns_a301a2-55 .kt-button{font-weight:normal;font-style:normal;}.kt-btns_a301a2-55 .kt-btn-wrap-0{margin-right:5px;}.wp-block-kadence-advancedbtn.kt-btns_a301a2-55 .kt-btn-wrap-0 .kt-button{color:#fff;background:#009900;border-color:#fff;box-shadow:1px 1px 2px 3px rgba(0, 0, 0, 0.2);}.wp-block-kadence-advancedbtn.kt-btns_a301a2-55 .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns_a301a2-55 .kt-btn-wrap-0 .kt-button:focus{color:#ffffff;border-color:#444444;}.wp-block-kadence-advancedbtn.kt-btns_a301a2-55 .kt-btn-wrap-0 .kt-button::before{display:none;}.wp-block-kadence-advancedbtn.kt-btns_a301a2-55 .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns_a301a2-55 .kt-btn-wrap-0 .kt-button:focus{background:#444444;}<\/style>\n<div class=\"wp-block-kadence-advancedbtn kt-btn-align-center kt-btn-tablet-align-inherit kt-btn-mobile-align-inherit kt-btns-wrap kt-btns_a301a2-55\"><div class=\"kt-btn-wrap kt-btn-wrap-0\"><a class=\"kt-button kt-btn-0-action kt-btn-size-standard kt-btn-style-basic kt-btn-svg-show-always kt-btn-has-text-true kt-btn-has-svg-false\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35440493\/\"><span class=\"kt-btn-inner-text\">Read the whole story<\/span><\/a><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Abstract: Luciferase reporter assays represent a simple and sensitive experimental system in cell and molecular biology to study multiple biological processes. However, the application of these assays is often limited by the costs of conventional luminometer instruments and the versatility &hellip; <a href=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/luminocell-a-versatile-and-affordable-platform-for-real-time-monitoring-of-luciferase-based-reporters\/\">Read More<\/a><\/p>\n","protected":false},"author":5,"featured_media":9937,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"kt_blocks_editor_width":"","_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[412,252],"tags":[304],"class_list":["post-9936","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bryjalab","category-nova-publikace","tag-bryjalab"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.1.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters - Department of Animal Physiology and Immunology<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/luminocell-a-versatile-and-affordable-platform-for-real-time-monitoring-of-luciferase-based-reporters\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters - Department of Animal Physiology and Immunology\" \/>\n<meta property=\"og:description\" content=\"Abstract: Luciferase reporter assays represent a simple and sensitive experimental system in cell and molecular biology to study multiple biological processes. However, the application of these assays is often limited by the costs of conventional luminometer instruments and the versatility &hellip; Read More\" \/>\n<meta property=\"og:url\" content=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/luminocell-a-versatile-and-affordable-platform-for-real-time-monitoring-of-luciferase-based-reporters\/\" \/>\n<meta property=\"og:site_name\" content=\"Department of Animal Physiology and Immunology\" \/>\n<meta property=\"article:published_time\" content=\"2022-04-25T17:14:43+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2022-12-01T17:15:52+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/luminocell.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"679\" \/>\n\t<meta property=\"og:image:height\" content=\"841\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Lucie Nesvadbov\u00e1\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Lucie Nesvadbov\u00e1\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/luminocell-a-versatile-and-affordable-platform-for-real-time-monitoring-of-luciferase-based-reporters\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/luminocell-a-versatile-and-affordable-platform-for-real-time-monitoring-of-luciferase-based-reporters\/\"},\"author\":{\"name\":\"Lucie Nesvadbov\u00e1\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/person\/3119766c8e4c78f08bbbde220469aee6\"},\"headline\":\"LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters\",\"datePublished\":\"2022-04-25T17:14:43+00:00\",\"dateModified\":\"2022-12-01T17:15:52+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/luminocell-a-versatile-and-affordable-platform-for-real-time-monitoring-of-luciferase-based-reporters\/\"},\"wordCount\":304,\"publisher\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#organization\"},\"image\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/luminocell-a-versatile-and-affordable-platform-for-real-time-monitoring-of-luciferase-based-reporters\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2022\/12\/luminocell.jpg\",\"keywords\":[\"Bryjalab\"],\"articleSection\":[\"Bryjalab\",\"Nov\u00e1 publikace\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/luminocell-a-versatile-and-affordable-platform-for-real-time-monitoring-of-luciferase-based-reporters\/\",\"url\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/luminocell-a-versatile-and-affordable-platform-for-real-time-monitoring-of-luciferase-based-reporters\/\",\"name\":\"LuminoCell: a versatile and affordable platform for real-time monitoring of luciferase-based reporters - 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