{"id":7880,"date":"2021-03-18T11:08:08","date_gmt":"2021-03-18T11:08:08","guid":{"rendered":"http:\/\/www.sci.muni.cz\/ofiz\/?p=7880"},"modified":"2021-03-18T11:09:30","modified_gmt":"2021-03-18T11:09:30","slug":"duct-reveals-architectural-mechanisms-contributing-to-bile-duct-recovery-in-a-mouse-model-for-alagille-syndrome-2","status":"publish","type":"post","link":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/duct-reveals-architectural-mechanisms-contributing-to-bile-duct-recovery-in-a-mouse-model-for-alagille-syndrome-2\/","title":{"rendered":"DUCT reveals architectural mechanisms contributing to bile duct recovery in a mouse model for Alagille syndrome"},"content":{"rendered":"\n
Abstract:<\/strong><\/h5>\n

Organ function depends on tissues adopting the correct architecture. However, insights into organ architecture are currently hampered by an absence of standardized quantitative 3D analysis.<\/p>\n

We aimed to develop a robust technology to visualize, digitalize, and segment the architecture of two tubular systems in 3D: double resin casting micro computed tomography (DUCT).<\/p>\n

As proof of principle, we applied DUCT to a mouse model for Alagille syndrome (Jag1Ndr\/Ndr mice), characterized by intrahepatic bile duct paucity, that can spontaneously generate a biliary system in adulthood. DUCT identified increased central biliary branching and peripheral bile duct tortuosity as two compensatory processes occurring in distinct regions of Jag1Ndr\/Ndr liver, leading to full reconstitution of wild-type biliary volume and phenotypic recovery.<\/p>\n

DUCT is thus a powerful new technology for 3D analysis, which can reveal novel phenotypes and provide a standardized method of defining liver architecture in mouse models.<\/p>\n\n\n\n

Elife. 2021 Feb 26;10:e60916.doi: 10.7554\/eLife.60916.<\/a> <\/em><\/p>\n\n\n\n

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Authors:<\/strong><\/h5>\n\n\n\n

Simona Hankeova<\/strong> #1,2<\/sup>, Jakub Salplachta #3<\/sup>, Tomas Zikmund #3<\/sup>, Michaela Kavkova #3<\/sup>, No\u00e9mi Van Hul #1<\/sup>, Adam Brinek 3<\/sup>, Veronika Smekalova 3<\/sup>, Jakub Laznovsky 3<\/sup>, Feven Dawit 4<\/sup>, Josef Jaros 5<\/sup>, V\u00edt\u011bzslav Bryja<\/strong> 2<\/sup>, Urban Lendahl 6<\/sup>, Ewa Ellis 4<\/sup>, Antal Nemeth 7<\/sup>, Bj\u00f6rn Fischler 4<\/sup>, Edouard Hannezo 8<\/sup>, Jozef Kaiser 3<\/sup>, Emma Rachel Andersson 1,6<\/sup><\/p>\n

<\/p>\n

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  • 1<\/sup> Department of Biosciences and Nutrition, Karolinska Institutet, Solna, Sweden.<\/li>\n
  • 2<\/sup> Department of Experimental Biology, Masaryk University, Brno, Czech Republic.<\/strong><\/li>\n
  • 3<\/sup> CEITEC – Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.<\/li>\n
  • 4<\/sup> Department of Pediatrics, Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet and Karolinska University Hospital, Solna, Sweden.<\/li>\n
  • 5<\/sup> Department of Histology and Embryology, Masaryk University, Brno, Czech Republic.<\/li>\n
  • 6<\/sup> Department of Cell and Molecular Biology, Karolinska Institutet, Solna, Sweden.<\/li>\n
  • 7<\/sup> Department of Laboratory Medicine, Karolinska Institutet, Solna, Sweden.<\/li>\n
  • 8<\/sup> Institute of Science and Technology, Klosterneuburg, Austria.<\/li>\n<\/ul>\n

    #<\/sup> Contributed equally.<\/p>\n\n\n