{"id":6542,"date":"2020-10-08T07:43:01","date_gmt":"2020-10-08T07:43:01","guid":{"rendered":"http:\/\/www.sci.muni.cz\/ofiz\/?p=6542"},"modified":"2020-10-08T07:43:03","modified_gmt":"2020-10-08T07:43:03","slug":"12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2","status":"publish","type":"post","link":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/","title":{"rendered":"12-O-Tetradecanoylphorbol-13-acetate increases cardiomyogenesis through PKC\/ERK signaling"},"content":{"rendered":"\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"484\" src=\"https:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western-1024x484.jpg\" alt=\"\" class=\"wp-image-6535\" srcset=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western-1024x484.jpg 1024w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western-300x142.jpg 300w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western-768x363.jpg 768w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western-848x401.jpg 848w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western-560x265.jpg 560w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western-740x350.jpg 740w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western-370x175.jpg 370w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western.jpg 1388w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption><em><strong>The role of PKC and ERK signalling pathways in TPA-induced cardiomyogenesis<\/strong><\/em>: Western blot analysis of the<br> phosphorylation status of PKC, ERK, and GATA4 and the level of EGR1 after the treatment of R1 cell-derived 6-day-old embryoid  bodies with 0.1% DMSO or 1 \u03bcM TPA and the aforementioned inhibitors in the indicated time intervals.<\/figcaption><\/figure>\n\n\n\n<p style=\"text-align: justify;\">12-O-Tetradecanoylphorbol-13-acetate (TPA) is the most widely used diacylglycerol (DAG) mimetic agent and inducer of protein kinase C (PKC)-mediated cellular response in biomedical studies. TPA has been proposed as a pluripotent cell differentiation factor, but results obtained have been inconsistent. In the present study we show that TPA can be applied as a cardiomyogenesis-promoting factor for the differentiation of mouse embryonic stem (mES) cells in vitro. The mechanism of TPA action is mediated by the induction of extracellular signal-regulated kinase (ERK) activity and the subsequent phosphorylation of GATA4 transcription factor. Interestingly, general mitogens (FGF, EGF, VEGF and serum) or canonical WNT signalling did not mimic the effect of TPA. Moreover, on the basis of our results, we postulate that a TPA-sensitive population of cardiac progenitor cells exists at a certain time point (after days 6\u20138 of the differentiation protocol) and that the proposed treatment can be used to increase the multiplication of ES cell-derived cardiomyocytes.<\/p>\n\n\n\n<p style=\"text-align: right;\"><a href=\"https:\/\/www.nature.com\/srep\" data-test=\"journal-link\"><i data-test=\"journal-title\">Scientific Reports<\/i><\/a>&nbsp;<span class=\"u-visually-hidden\">volume<\/span>&nbsp;10, Article&nbsp;number:&nbsp;<span data-test=\"article-number\">15922<\/span>&nbsp;(<span data-test=\"article-publication-year\">2020<\/span>)&nbsp;<\/p>\n\n\n\n<p>by<\/p>\n\n\n\n<h5><strong>Katarzyna Anna Radaszkiewicz, Deborah Beckerov\u00e1, Lucie Woloszczukov\u00e1, Tomasz Witold Radaszkiewicz, Petra Les\u00e1kov\u00e1, Olga Vond\u00e1lov\u00e1 Blan\u00e1\u0159ov\u00e1, Luk\u00e1\u0161 Kubala,<\/strong> Petr Humpol\u00ed\u010dek <strong>&amp; Ji\u0159\u00ed Pachernik<\/strong><\/h5>\n\n\n<style>.wp-block-kadence-advancedbtn.kb-btns_33a792-d1{gap:var(--global-kb-gap-xs, 0.5rem );justify-content:center;align-items:center;}.kt-btns_33a792-d1 .kt-button{font-weight:normal;font-style:normal;}.kt-btns_33a792-d1 .kt-btn-wrap-0{margin-right:5px;}.wp-block-kadence-advancedbtn.kt-btns_33a792-d1 .kt-btn-wrap-0 .kt-button{color:#fff;background:#009900;border-color:#fff;box-shadow:1px 1px 2px 3px rgba(0, 0, 0, 0.2);}.wp-block-kadence-advancedbtn.kt-btns_33a792-d1 .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns_33a792-d1 .kt-btn-wrap-0 .kt-button:focus{color:#ffffff;border-color:#444444;}.wp-block-kadence-advancedbtn.kt-btns_33a792-d1 .kt-btn-wrap-0 .kt-button::before{display:none;}.wp-block-kadence-advancedbtn.kt-btns_33a792-d1 .kt-btn-wrap-0 .kt-button:hover, .wp-block-kadence-advancedbtn.kt-btns_33a792-d1 .kt-btn-wrap-0 .kt-button:focus{background:#444444;}<\/style>\n<div class=\"wp-block-kadence-advancedbtn kt-btn-align-center kt-btn-tablet-align-inherit kt-btn-mobile-align-inherit kt-btns-wrap kt-btns_33a792-d1\"><div class=\"kt-btn-wrap kt-btn-wrap-0\"><a class=\"kt-button kt-btn-0-action kt-btn-size-standard kt-btn-style-basic kt-btn-svg-show-always kt-btn-has-text-true kt-btn-has-svg-false\" href=\"https:\/\/www.nature.com\/articles\/s41598-020-73074-4\"><span class=\"kt-btn-inner-text\">Read the whole story<\/span><\/a><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>12-O-Tetradecanoylphorbol-13-acetate (TPA) is the most widely used diacylglycerol (DAG) mimetic agent and inducer of protein kinase C (PKC)-mediated cellular response in biomedical studies. TPA has been proposed as a pluripotent cell differentiation factor, but results obtained have been inconsistent. In &hellip; <a href=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/\">Read More<\/a><\/p>\n","protected":false},"author":5,"featured_media":6535,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"kt_blocks_editor_width":"","_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[256],"tags":[],"class_list":["post-6542","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-new-paper"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.1.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>12-O-Tetradecanoylphorbol-13-acetate increases cardiomyogenesis through PKC\/ERK signaling - Department of Animal Physiology and Immunology<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"12-O-Tetradecanoylphorbol-13-acetate increases cardiomyogenesis through PKC\/ERK signaling - Department of Animal Physiology and Immunology\" \/>\n<meta property=\"og:description\" content=\"12-O-Tetradecanoylphorbol-13-acetate (TPA) is the most widely used diacylglycerol (DAG) mimetic agent and inducer of protein kinase C (PKC)-mediated cellular response in biomedical studies. TPA has been proposed as a pluripotent cell differentiation factor, but results obtained have been inconsistent. In &hellip; Read More\" \/>\n<meta property=\"og:url\" content=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/\" \/>\n<meta property=\"og:site_name\" content=\"Department of Animal Physiology and Immunology\" \/>\n<meta property=\"article:published_time\" content=\"2020-10-08T07:43:01+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2020-10-08T07:43:03+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1388\" \/>\n\t<meta property=\"og:image:height\" content=\"656\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Lucie Nesvadbov\u00e1\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Lucie Nesvadbov\u00e1\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/\"},\"author\":{\"name\":\"Lucie Nesvadbov\u00e1\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/person\/3119766c8e4c78f08bbbde220469aee6\"},\"headline\":\"12-O-Tetradecanoylphorbol-13-acetate increases cardiomyogenesis through PKC\/ERK signaling\",\"datePublished\":\"2020-10-08T07:43:01+00:00\",\"dateModified\":\"2020-10-08T07:43:03+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/\"},\"wordCount\":256,\"publisher\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#organization\"},\"image\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western.jpg\",\"articleSection\":[\"New paper\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/\",\"url\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/\",\"name\":\"12-O-Tetradecanoylphorbol-13-acetate increases cardiomyogenesis through PKC\/ERK signaling - Department of Animal Physiology and Immunology\",\"isPartOf\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western.jpg\",\"datePublished\":\"2020-10-08T07:43:01+00:00\",\"dateModified\":\"2020-10-08T07:43:03+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#primaryimage\",\"url\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western.jpg\",\"contentUrl\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western.jpg\",\"width\":1388,\"height\":656},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"12-O-Tetradecanoylphorbol-13-acetate increases cardiomyogenesis through PKC\/ERK signaling\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#website\",\"url\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/\",\"name\":\"Department of Animal Physiology and Immunology\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#organization\",\"name\":\"Department of Animal Physiology and Immunology\",\"url\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/02\/logo_EN.png\",\"contentUrl\":\"https:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/02\/logo_EN.png\",\"width\":1903,\"height\":264,\"caption\":\"Department of Animal Physiology and Immunology\"},\"image\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/logo\/image\/\"}},{\"@type\":\"Person\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/person\/3119766c8e4c78f08bbbde220469aee6\",\"name\":\"Lucie Nesvadbov\u00e1\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/a91adf81f15a662a55b84fac48c8a8a4f22879e0ab9141bb8487b53bb898b6a6?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/a91adf81f15a662a55b84fac48c8a8a4f22879e0ab9141bb8487b53bb898b6a6?s=96&d=mm&r=g\",\"caption\":\"Lucie Nesvadbov\u00e1\"},\"url\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/author\/nesvadbova\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"12-O-Tetradecanoylphorbol-13-acetate increases cardiomyogenesis through PKC\/ERK signaling - Department of Animal Physiology and Immunology","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/","og_locale":"en_US","og_type":"article","og_title":"12-O-Tetradecanoylphorbol-13-acetate increases cardiomyogenesis through PKC\/ERK signaling - Department of Animal Physiology and Immunology","og_description":"12-O-Tetradecanoylphorbol-13-acetate (TPA) is the most widely used diacylglycerol (DAG) mimetic agent and inducer of protein kinase C (PKC)-mediated cellular response in biomedical studies. TPA has been proposed as a pluripotent cell differentiation factor, but results obtained have been inconsistent. In &hellip; Read More","og_url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/","og_site_name":"Department of Animal Physiology and Immunology","article_published_time":"2020-10-08T07:43:01+00:00","article_modified_time":"2020-10-08T07:43:03+00:00","og_image":[{"width":1388,"height":656,"url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western.jpg","type":"image\/jpeg"}],"author":"Lucie Nesvadbov\u00e1","twitter_card":"summary_large_image","twitter_misc":{"Written by":"Lucie Nesvadbov\u00e1","Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#article","isPartOf":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/"},"author":{"name":"Lucie Nesvadbov\u00e1","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/person\/3119766c8e4c78f08bbbde220469aee6"},"headline":"12-O-Tetradecanoylphorbol-13-acetate increases cardiomyogenesis through PKC\/ERK signaling","datePublished":"2020-10-08T07:43:01+00:00","dateModified":"2020-10-08T07:43:03+00:00","mainEntityOfPage":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/"},"wordCount":256,"publisher":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#organization"},"image":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#primaryimage"},"thumbnailUrl":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western.jpg","articleSection":["New paper"],"inLanguage":"en-US"},{"@type":"WebPage","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/","url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/","name":"12-O-Tetradecanoylphorbol-13-acetate increases cardiomyogenesis through PKC\/ERK signaling - Department of Animal Physiology and Immunology","isPartOf":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#website"},"primaryImageOfPage":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#primaryimage"},"image":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#primaryimage"},"thumbnailUrl":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western.jpg","datePublished":"2020-10-08T07:43:01+00:00","dateModified":"2020-10-08T07:43:03+00:00","breadcrumb":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#primaryimage","url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western.jpg","contentUrl":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/10\/western.jpg","width":1388,"height":656},{"@type":"BreadcrumbList","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/12-o-tetradecanoylphorbol-13-acetate-increases-cardiomyogenesis-through-pkc-erk-signaling-2\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/ueb1.sci.muni.cz\/ofiz\/"},{"@type":"ListItem","position":2,"name":"12-O-Tetradecanoylphorbol-13-acetate increases cardiomyogenesis through PKC\/ERK signaling"}]},{"@type":"WebSite","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#website","url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/","name":"Department of Animal Physiology and Immunology","description":"","publisher":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/ueb1.sci.muni.cz\/ofiz\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#organization","name":"Department of Animal Physiology and Immunology","url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/logo\/image\/","url":"https:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/02\/logo_EN.png","contentUrl":"https:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/02\/logo_EN.png","width":1903,"height":264,"caption":"Department of Animal Physiology and Immunology"},"image":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/logo\/image\/"}},{"@type":"Person","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/person\/3119766c8e4c78f08bbbde220469aee6","name":"Lucie Nesvadbov\u00e1","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/a91adf81f15a662a55b84fac48c8a8a4f22879e0ab9141bb8487b53bb898b6a6?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/a91adf81f15a662a55b84fac48c8a8a4f22879e0ab9141bb8487b53bb898b6a6?s=96&d=mm&r=g","caption":"Lucie Nesvadbov\u00e1"},"url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/author\/nesvadbova\/"}]}},"_links":{"self":[{"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/posts\/6542","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/comments?post=6542"}],"version-history":[{"count":2,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/posts\/6542\/revisions"}],"predecessor-version":[{"id":7124,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/posts\/6542\/revisions\/7124"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/media\/6535"}],"wp:attachment":[{"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/media?parent=6542"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/categories?post=6542"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/tags?post=6542"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}