{"id":3563,"date":"2019-11-19T07:45:28","date_gmt":"2019-11-19T07:45:28","guid":{"rendered":"http:\/\/www.sci.muni.cz\/ofiz\/?p=3563"},"modified":"2019-11-19T07:47:58","modified_gmt":"2019-11-19T07:47:58","slug":"the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice","status":"publish","type":"post","link":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/","title":{"rendered":"The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice"},"content":{"rendered":"\n<p>Although the production of engineered nanoparticles increases our knowledge of toxicity and mechanisms of bioactivity during relevant exposures is lacking. In the present study, mice were exposed to PbO nanoparticles (PbONP; 192.5\u2009\u00b5g\/m<sup>3<\/sup>; 1.93\u2009\u00d7\u200910<sup>6<\/sup> particles\/cm<sup>3<\/sup>)  for 2, 5 and 13\u2009weeks through continuous inhalation. The analyses addressed Pb and PbONP distribution in organs (lung, liver, kidney,  brain) using electrothermal atomic absorption spectrometry and transmission electron microscopy, as well as histopathology and analyses of oxidative stress biomarkers. New LC-MS\/MS methods were validated for biomarkers of lipid damage F2-isoprostanes (8-iso-prostaglandins F2-alpha and E2)  and hydroxylated deoxoguanosine (8-OHdG, a marker of DNA oxidation). Commonly studied malondialdehyde was also measured as TBARS by HPLC-DAD.  The study revealed fast blood transport and distribution of Pb from the lung to the kidney and liver. A different Pb accumulation trend was observed in the brain, suggesting the transfer of NP along the nasal nerve to the olfactory bulbs. Long-term inhalation of PbONP caused lipid peroxidation in animal brains (increased levels of TBARS and both isoprostanes). Membrane lipid damage was also detected in the kidney after shorter exposures, but not in the liver or lung. On the contrary,  longer exposures to PbONP increased levels of 8-OHdG in the lung and temporarily increased lung weight after 2 and 5\u2009weeks of exposure. The histopathological changes observed mainly in the lung and liver indicated inflammation and general toxicity responses. The present long-term inhalation study indicates risks of PbONP to both human health and the environment. <\/p>\n\n\n\n<p>Cel\u00fd \u010dl\u00e1nek si m\u016f\u017eete p\u0159e\u010d\u00edst <a href=\"https:\/\/www.tandfonline.com\/doi\/abs\/10.1080\/17435390.2019.1685696?needAccess=true#aHR0cHM6Ly93d3cudGFuZGZvbmxpbmUuY29tL2RvaS9wZGYvMTAuMTA4MC8xNzQzNTM5MC4yMDE5LjE2ODU2OTY\/bmVlZEFjY2Vzcz10cnVlQEBAMA==\">tady<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Although the production of engineered nanoparticles increases our knowledge of toxicity and mechanisms of bioactivity during relevant exposures is lacking. In the present study, mice were exposed to PbO nanoparticles (PbONP; 192.5\u2009\u00b5g\/m3; 1.93\u2009\u00d7\u2009106 particles\/cm3) for 2, 5 and 13\u2009weeks through &hellip; <a href=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/\">Read More<\/a><\/p>\n","protected":false},"author":5,"featured_media":3564,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"kt_blocks_editor_width":"","_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[252],"tags":[307],"class_list":["post-3563","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-nova-publikace","tag-buchtova-lab"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.1.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice - Department of Animal Physiology and Immunology<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice - Department of Animal Physiology and Immunology\" \/>\n<meta property=\"og:description\" content=\"Although the production of engineered nanoparticles increases our knowledge of toxicity and mechanisms of bioactivity during relevant exposures is lacking. In the present study, mice were exposed to PbO nanoparticles (PbONP; 192.5\u2009\u00b5g\/m3; 1.93\u2009\u00d7\u2009106 particles\/cm3) for 2, 5 and 13\u2009weeks through &hellip; Read More\" \/>\n<meta property=\"og:url\" content=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/\" \/>\n<meta property=\"og:site_name\" content=\"Department of Animal Physiology and Immunology\" \/>\n<meta property=\"article:published_time\" content=\"2019-11-19T07:45:28+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2019-11-19T07:47:58+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/11\/Marci_clanek_2019_11.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1048\" \/>\n\t<meta property=\"og:image:height\" content=\"372\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Lucie Nesvadbov\u00e1\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Lucie Nesvadbov\u00e1\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/\"},\"author\":{\"name\":\"Lucie Nesvadbov\u00e1\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/person\/3119766c8e4c78f08bbbde220469aee6\"},\"headline\":\"The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice\",\"datePublished\":\"2019-11-19T07:45:28+00:00\",\"dateModified\":\"2019-11-19T07:47:58+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/\"},\"wordCount\":273,\"publisher\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#organization\"},\"image\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/11\/Marci_clanek_2019_11.jpg\",\"keywords\":[\"Buchtova lab\"],\"articleSection\":[\"Nov\u00e1 publikace\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/\",\"url\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/\",\"name\":\"The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice - Department of Animal Physiology and Immunology\",\"isPartOf\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/11\/Marci_clanek_2019_11.jpg\",\"datePublished\":\"2019-11-19T07:45:28+00:00\",\"dateModified\":\"2019-11-19T07:47:58+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#primaryimage\",\"url\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/11\/Marci_clanek_2019_11.jpg\",\"contentUrl\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/11\/Marci_clanek_2019_11.jpg\",\"width\":1048,\"height\":372},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#website\",\"url\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/\",\"name\":\"Department of Animal Physiology and Immunology\",\"description\":\"\",\"publisher\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#organization\",\"name\":\"Department of Animal Physiology and Immunology\",\"url\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/logo\/image\/\",\"url\":\"https:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/02\/logo_EN.png\",\"contentUrl\":\"https:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/02\/logo_EN.png\",\"width\":1903,\"height\":264,\"caption\":\"Department of Animal Physiology and Immunology\"},\"image\":{\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/logo\/image\/\"}},{\"@type\":\"Person\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/person\/3119766c8e4c78f08bbbde220469aee6\",\"name\":\"Lucie Nesvadbov\u00e1\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/secure.gravatar.com\/avatar\/a91adf81f15a662a55b84fac48c8a8a4f22879e0ab9141bb8487b53bb898b6a6?s=96&d=mm&r=g\",\"contentUrl\":\"https:\/\/secure.gravatar.com\/avatar\/a91adf81f15a662a55b84fac48c8a8a4f22879e0ab9141bb8487b53bb898b6a6?s=96&d=mm&r=g\",\"caption\":\"Lucie Nesvadbov\u00e1\"},\"url\":\"https:\/\/ueb1.sci.muni.cz\/ofiz\/author\/nesvadbova\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice - Department of Animal Physiology and Immunology","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/","og_locale":"en_US","og_type":"article","og_title":"The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice - Department of Animal Physiology and Immunology","og_description":"Although the production of engineered nanoparticles increases our knowledge of toxicity and mechanisms of bioactivity during relevant exposures is lacking. In the present study, mice were exposed to PbO nanoparticles (PbONP; 192.5\u2009\u00b5g\/m3; 1.93\u2009\u00d7\u2009106 particles\/cm3) for 2, 5 and 13\u2009weeks through &hellip; Read More","og_url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/","og_site_name":"Department of Animal Physiology and Immunology","article_published_time":"2019-11-19T07:45:28+00:00","article_modified_time":"2019-11-19T07:47:58+00:00","og_image":[{"width":1048,"height":372,"url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/11\/Marci_clanek_2019_11.jpg","type":"image\/jpeg"}],"author":"Lucie Nesvadbov\u00e1","twitter_card":"summary_large_image","twitter_misc":{"Written by":"Lucie Nesvadbov\u00e1","Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#article","isPartOf":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/"},"author":{"name":"Lucie Nesvadbov\u00e1","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/person\/3119766c8e4c78f08bbbde220469aee6"},"headline":"The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice","datePublished":"2019-11-19T07:45:28+00:00","dateModified":"2019-11-19T07:47:58+00:00","mainEntityOfPage":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/"},"wordCount":273,"publisher":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#organization"},"image":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#primaryimage"},"thumbnailUrl":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/11\/Marci_clanek_2019_11.jpg","keywords":["Buchtova lab"],"articleSection":["Nov\u00e1 publikace"],"inLanguage":"en-US"},{"@type":"WebPage","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/","url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/","name":"The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice - Department of Animal Physiology and Immunology","isPartOf":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#website"},"primaryImageOfPage":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#primaryimage"},"image":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#primaryimage"},"thumbnailUrl":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/11\/Marci_clanek_2019_11.jpg","datePublished":"2019-11-19T07:45:28+00:00","dateModified":"2019-11-19T07:47:58+00:00","breadcrumb":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#primaryimage","url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/11\/Marci_clanek_2019_11.jpg","contentUrl":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/11\/Marci_clanek_2019_11.jpg","width":1048,"height":372},{"@type":"BreadcrumbList","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/the-effects-of-nano-sized-pbo-on-biomarkers-of-membrane-disruption-and-dna-damage-in-a-sub-chronic-inhalation-study-on-mice\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/ueb1.sci.muni.cz\/ofiz\/"},{"@type":"ListItem","position":2,"name":"The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice"}]},{"@type":"WebSite","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#website","url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/","name":"Department of Animal Physiology and Immunology","description":"","publisher":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/ueb1.sci.muni.cz\/ofiz\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#organization","name":"Department of Animal Physiology and Immunology","url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/logo\/image\/","url":"https:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/02\/logo_EN.png","contentUrl":"https:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2019\/02\/logo_EN.png","width":1903,"height":264,"caption":"Department of Animal Physiology and Immunology"},"image":{"@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/logo\/image\/"}},{"@type":"Person","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/person\/3119766c8e4c78f08bbbde220469aee6","name":"Lucie Nesvadbov\u00e1","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/ueb1.sci.muni.cz\/ofiz\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/a91adf81f15a662a55b84fac48c8a8a4f22879e0ab9141bb8487b53bb898b6a6?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/a91adf81f15a662a55b84fac48c8a8a4f22879e0ab9141bb8487b53bb898b6a6?s=96&d=mm&r=g","caption":"Lucie Nesvadbov\u00e1"},"url":"https:\/\/ueb1.sci.muni.cz\/ofiz\/author\/nesvadbova\/"}]}},"_links":{"self":[{"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/posts\/3563","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/comments?post=3563"}],"version-history":[{"count":2,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/posts\/3563\/revisions"}],"predecessor-version":[{"id":3568,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/posts\/3563\/revisions\/3568"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/media\/3564"}],"wp:attachment":[{"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/media?parent=3563"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/categories?post=3563"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-json\/wp\/v2\/tags?post=3563"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}