{"id":2123,"date":"2019-06-07T13:13:26","date_gmt":"2019-06-07T13:13:26","guid":{"rendered":"http:\/\/www.sci.muni.cz\/ofiz\/?p=2123"},"modified":"2019-06-07T13:13:27","modified_gmt":"2019-06-07T13:13:27","slug":"dishevelled-3-conformation-dynamics-analyzed-by-fret-based-biosensors-reveals-a-key-role-of-casein-kinase-1","status":"publish","type":"post","link":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/dishevelled-3-conformation-dynamics-analyzed-by-fret-based-biosensors-reveals-a-key-role-of-casein-kinase-1\/","title":{"rendered":"Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1"},"content":{"rendered":"\n

Dishevelled (DVL) is the key component \nof the Wnt signaling pathway. Currently, DVL conformational dynamics \nunder native conditions is unknown. To overcome this limitation, we \ndevelop the Fluorescein Arsenical Hairpin Binder- (FlAsH-) based FRET in\n vivo approach to study DVL conformation in living cells. Using this \nsingle-cell FRET approach, we demonstrate that (i) Wnt ligands induce \nopen DVL conformation, (ii) DVL variants that are predominantly open, \nshow more even subcellular localization and more efficient membrane \nrecruitment by Frizzled (FZD) and (iii) Casein kinase 1 \u025b (CK1\u025b) has a \nkey regulatory function in DVL conformational dynamics. In silico \nmodeling and in vitro biophysical methods explain how CK1\u025b-specific \nphosphorylation events control DVL conformations via modulation of the \nPDZ domain and its interaction with DVL C-terminus. In summary, our \nstudy describes an experimental tool for DVL conformational sampling in \nliving cells and elucidates the essential regulatory role of CK1\u025b in DVL\n conformational dynamics.<\/p>\n\n\n\n

More information<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Dishevelled (DVL) is the key component of the Wnt signaling pathway. Currently, DVL conformational dynamics under native conditions is unknown. To overcome this limitation, we develop the Fluorescein Arsenical Hairpin Binder- (FlAsH-) based FRET in vivo approach to study DVL … Read More<\/a><\/p>\n","protected":false},"author":5,"featured_media":2120,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"kt_blocks_editor_width":"","_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[256],"tags":[],"class_list":["post-2123","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-new-paper"],"yoast_head":"\nDishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1 - Department of Animal Physiology and Immunology<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/dishevelled-3-conformation-dynamics-analyzed-by-fret-based-biosensors-reveals-a-key-role-of-casein-kinase-1\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1 - Department of Animal Physiology and Immunology\" \/>\n<meta property=\"og:description\" content=\"Dishevelled (DVL) is the key component of the Wnt signaling pathway. Currently, DVL conformational dynamics under native conditions is unknown. 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