{"id":668,"date":"2019-05-23T19:35:15","date_gmt":"2019-05-23T19:35:15","guid":{"rendered":"http:\/\/www.med.muni.cz\/histology\/ofiz\/?page_id=668"},"modified":"2023-09-01T19:14:10","modified_gmt":"2023-09-01T19:14:10","slug":"bryja","status":"publish","type":"page","link":"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/bryja\/","title":{"rendered":"V\u00edt\u011bzslav Bryja"},"content":{"rendered":"<style>#kt-layout-id_97cbf3-10 > .kt-row-column-wrap{align-content:start;}:where(#kt-layout-id_97cbf3-10 > .kt-row-column-wrap) > .wp-block-kadence-column{justify-content:start;}#kt-layout-id_97cbf3-10 > .kt-row-column-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:var(--global-kb-gap-md, 2rem);padding-top:var( --global-kb-row-default-top, 25px );padding-bottom:var( --global-kb-row-default-bottom, 25px );padding-top:0px;padding-bottom:0px;grid-template-columns:minmax(0, calc(70% - ((var(--global-kb-gap-md, 2rem) * 1 )\/2)))minmax(0, calc(30% - ((var(--global-kb-gap-md, 2rem) * 1 )\/2)));}#kt-layout-id_97cbf3-10 > .kt-row-layout-overlay{opacity:0.30;}@media all and (max-width: 1024px){#kt-layout-id_97cbf3-10 > .kt-row-column-wrap{grid-template-columns:minmax(0, 2fr) minmax(0, 1fr);}}@media all and (max-width: 767px){#kt-layout-id_97cbf3-10 > .kt-row-column-wrap{grid-template-columns:minmax(0, 1fr);}}<\/style>\n<div class=\"wp-block-kadence-rowlayout alignnone\"><div id=\"kt-layout-id_97cbf3-10\" class=\"kt-row-layout-inner  kt-layout-id_97cbf3-10\"><div class=\"kt-row-column-wrap kt-has-2-columns kt-gutter-default kt-v-gutter-default kt-row-valign-top kt-row-layout-left-golden kt-tab-layout-inherit kt-m-colapse-left-to-right kt-mobile-layout-row kt-custom-first-width-70 kt-custom-second-width-30\"><style>.kadence-column_01d564-44 > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_01d564-44 > .kt-inside-inner-col,.kadence-column_01d564-44 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_01d564-44 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_01d564-44 > .kt-inside-inner-col{flex-direction:column;}.kadence-column_01d564-44 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_01d564-44 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_01d564-44{position:relative;}@media all and (max-width: 1024px){.kadence-column_01d564-44 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_01d564-44 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-1 kadence-column_01d564-44\"><div class=\"kt-inside-inner-col\">\n<h2><strong>V\u00edt\u011bzslav Bryja &#8211; Laboratory of Cellular Communication<\/strong><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">Introduction<\/h3>\n\n\n\n<p>Morphogenetic proteins from the&nbsp;<strong>Wnt family<\/strong>&nbsp;are important regulators of&nbsp;<strong>embryonal development<\/strong>. They have also huge impact on&nbsp;<strong>homeostasis regulation<\/strong>&nbsp;of the adult organism. Deregulation of Wnt pathway is associated with&nbsp;<strong>cancer and many other diseases<\/strong>. Although the significance of this family of proteins in the pathogenesis of diseases is widely accepted, we do not know much about the molecular mechanisms of their effect.<\/p>\n<\/div><\/div>\n\n\n<style>.kadence-column_8216e3-36 > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_8216e3-36 > .kt-inside-inner-col,.kadence-column_8216e3-36 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_8216e3-36 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_8216e3-36 > .kt-inside-inner-col{flex-direction:column;}.kadence-column_8216e3-36 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_8216e3-36 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_8216e3-36{position:relative;}@media all and (max-width: 1024px){.kadence-column_8216e3-36 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_8216e3-36 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-2 kadence-column_8216e3-36\"><div class=\"kt-inside-inner-col\">\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4717 aligncenter\" src=\"http:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Bryjalab_logo_kulate-300x300.png\" alt=\"\" width=\"184\" height=\"184\" srcset=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Bryjalab_logo_kulate-300x300.png 300w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Bryjalab_logo_kulate-150x150.png 150w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Bryjalab_logo_kulate-768x768.png 768w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Bryjalab_logo_kulate-1024x1024.png 1024w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Bryjalab_logo_kulate-60x60.png 60w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Bryjalab_logo_kulate.png 1125w\" sizes=\"auto, (max-width: 184px) 100vw, 184px\" \/><\/p>\n<\/div><\/div>\n<\/div><\/div><\/div>\n\n\n\n<p>Our laboratory attempts to contribute to understanding of some key molecular aspects of transduction of the Wnt signal between the<strong>&nbsp;Frizzled receptor<\/strong>, the protein&nbsp;<strong>Dishevelled (DVL)<\/strong>&nbsp;and other proteins in the Wnt signaling, mainly&nbsp;<strong>Casein kinase 1 epsilon (CK1e)<\/strong>. We believe these findings will become the basis for identification of new therapeutic targets for curing the tumors caused by the Wnt pathway deregulation.<\/p>\n\n\n\n\n\n<p>To study Wnt signaling we use all basic methods of<strong>&nbsp;biochemistry<\/strong>&nbsp;and&nbsp;<strong>molecular and cell biology<\/strong>&nbsp;and rely heavily on the most state-of-the-art&nbsp;<strong>proteomics and genomics<\/strong>. We frequently use the&nbsp;<strong>primary patient samples<\/strong>, which we obtain in cooperation with the Faculty Hospital Brno. Alone or in collaboration we are trying to take advantage of data validation&nbsp;<em>in vivo<\/em>&nbsp;using&nbsp;<em>Caenorhabditis elegans<\/em>,<em>&nbsp;Xenopus laevis<\/em>&nbsp;and&nbsp;<em>Mus musculus<\/em>&nbsp;as&nbsp;<strong>experimental models<\/strong>. <\/p>\n\n\n\n<div style=\"height:35px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3>Our research can be roughly classified into four, mutually interconnected, topics:<\/h3>\n\n\n\n<h4><strong>1) Biochemistry and biology of Dishevelled (DVL) protein<\/strong><\/h4>\n<p>Wnt proteins bind to the membrane receptors from the Frizzled family that transduce the signal onto the phosphoprotein&nbsp;<strong>Dishevelled (DVL)<\/strong>&nbsp;that directs signal towards one of several downstream signaling pathways. Molecular mechanisms determining how DVL performs its&nbsp;<strong>channeling function<\/strong>&nbsp;have not been discovered yet. It is believed that<strong>&nbsp;DVL phosphorylation, subcellular localization and conformation<\/strong>&nbsp;holds the key to understanding of its function. Using proteomics we attempt to&nbsp;<strong>crack DVL phosphorylation code<\/strong>, especially the code induced by&nbsp;<strong>Casein kinase 1 (CK1)<\/strong>. We implement many in-house derived unique tools and modern approaches (CRISPR-Cas9 knock-out technology, whole proteome analysis, FRET sensors etc.) to validate our findings functionally in the context of a living cell.<\/p>\n\n\n<style>#kt-layout-id_738785-4b > .kt-row-column-wrap{align-content:start;}:where(#kt-layout-id_738785-4b > .kt-row-column-wrap) > .wp-block-kadence-column{justify-content:start;}#kt-layout-id_738785-4b > .kt-row-column-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:var(--global-kb-gap-md, 2rem);padding-top:var( --global-kb-row-default-top, 25px );padding-bottom:var( --global-kb-row-default-bottom, 25px );padding-top:0px;grid-template-columns:minmax(0, calc(10% - ((var(--global-kb-gap-md, 2rem) * 2 )\/3)))minmax(0, calc(80% - ((var(--global-kb-gap-md, 2rem) * 2 )\/3)))minmax(0, calc(10% - ((var(--global-kb-gap-md, 2rem) * 2 )\/3)));}#kt-layout-id_738785-4b > .kt-row-layout-overlay{opacity:0.30;}@media all and (max-width: 1024px){#kt-layout-id_738785-4b > .kt-row-column-wrap > div:not(.added-for-specificity){grid-column:initial;}}@media all and (max-width: 1024px){#kt-layout-id_738785-4b > .kt-row-column-wrap{grid-template-columns:repeat(3, minmax(0, 1fr));}}@media all and (max-width: 767px){#kt-layout-id_738785-4b > .kt-row-column-wrap > div:not(.added-for-specificity){grid-column:initial;}#kt-layout-id_738785-4b > .kt-row-column-wrap{grid-template-columns:minmax(0, 1fr);}}<\/style>\n<div class=\"wp-block-kadence-rowlayout alignnone\"><div id=\"kt-layout-id_738785-4b\" class=\"kt-row-layout-inner  kt-layout-id_738785-4b\"><div class=\"kt-row-column-wrap kt-has-3-columns kt-gutter-default kt-v-gutter-default kt-row-valign-top kt-row-layout-equal kt-tab-layout-inherit kt-m-colapse-left-to-right kt-mobile-layout-row kt-custom-first-width-10 kt-custom-second-width-80 kt-custom-third-width-10\"><style>.kadence-column_5d7565-59 > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_5d7565-59 > .kt-inside-inner-col,.kadence-column_5d7565-59 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_5d7565-59 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_5d7565-59 > .kt-inside-inner-col{flex-direction:column;}.kadence-column_5d7565-59 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_5d7565-59 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_5d7565-59{position:relative;}@media all and (max-width: 1024px){.kadence-column_5d7565-59 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_5d7565-59 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-1 kadence-column_5d7565-59\"><div class=\"kt-inside-inner-col\">\n<p><\/p>\n<\/div><\/div>\n\n\n<style>.kadence-column_93cdf2-97 > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_93cdf2-97 > .kt-inside-inner-col,.kadence-column_93cdf2-97 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_93cdf2-97 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_93cdf2-97 > .kt-inside-inner-col{flex-direction:column;}.kadence-column_93cdf2-97 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_93cdf2-97 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_93cdf2-97{position:relative;}@media all and (max-width: 1024px){.kadence-column_93cdf2-97 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_93cdf2-97 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-2 kadence-column_93cdf2-97\"><div class=\"kt-inside-inner-col\">\n<div class=\"wp-block-image\"><figure class=\"aligncenter is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Research_1-1024x444.jpg\" alt=\"\" class=\"wp-image-4629\" width=\"654\" height=\"284\" srcset=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Research_1-1024x444.jpg 1024w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Research_1-300x130.jpg 300w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Research_1-768x333.jpg 768w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Research_1.jpg 1571w\" sizes=\"auto, (max-width: 654px) 100vw, 654px\" \/><\/figure><\/div>\n<\/div><\/div>\n\n\n<style>.kadence-column_d57b81-b4 > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_d57b81-b4 > .kt-inside-inner-col,.kadence-column_d57b81-b4 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_d57b81-b4 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_d57b81-b4 > .kt-inside-inner-col{flex-direction:column;}.kadence-column_d57b81-b4 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_d57b81-b4 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_d57b81-b4{position:relative;}@media all and (max-width: 1024px){.kadence-column_d57b81-b4 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_d57b81-b4 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-3 kadence-column_d57b81-b4\"><div class=\"kt-inside-inner-col\"><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n\n<h4><strong>2) Mechanistic understanding of the mammalian planar cell polarity (PCP) pathway<\/strong><\/h4>\n<p><strong>Cell polarization<\/strong>&nbsp;is a prerequisite for the control of cell shape, directional migration, asymmetric cell division and function of cells within complex tissues. One of the key pathways, which controls cell polarity and subsequently all the processes mentioned, is the&nbsp;<strong>non-canonical Wnt\/PCP (planar cell polarity) pathway<\/strong>. The Wnt\/PCP pathway&nbsp;<strong>controls migration and polarity<\/strong>&nbsp;in development of all multicellular organisms from cnidarians to men. Despite clear clinical importance of the Wnt\/PCP pathway, surprisingly little is known about the biochemical details of the signal transduction \u2013 this in turn prevents its efficient pharmacological inhibition\/activation in the pathological conditions. To reveal the biochemical changes associated with the activation of the PCP pathway we use the combination of a panel of PCP-deficient cell lines (generated by Crispr\/Cas9) and advanced proteomics (IP\/MS, BioID etc.). Subsequently, we functionally analyze how&nbsp;<strong>these mechanisms control asymmetric localization of PCP proteins<\/strong>&nbsp;in living cells and design screens for novel regulators which disrupt PCP signaling and&nbsp;<strong>block motility of migrating cancer cells<\/strong>.<\/p>\n\n\n<style>#kt-layout-id_e6b575-58 > .kt-row-column-wrap{align-content:start;}:where(#kt-layout-id_e6b575-58 > .kt-row-column-wrap) > .wp-block-kadence-column{justify-content:start;}#kt-layout-id_e6b575-58 > .kt-row-column-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:var(--global-kb-gap-md, 2rem);padding-top:var( --global-kb-row-default-top, 25px );padding-bottom:var( --global-kb-row-default-bottom, 25px );padding-top:0px;grid-template-columns:minmax(0, calc(15% - ((var(--global-kb-gap-md, 2rem) * 2 )\/3)))minmax(0, calc(75% - ((var(--global-kb-gap-md, 2rem) * 2 )\/3)))minmax(0, calc(10% - ((var(--global-kb-gap-md, 2rem) * 2 )\/3)));}#kt-layout-id_e6b575-58 > .kt-row-layout-overlay{opacity:0.30;}@media all and (max-width: 1024px){#kt-layout-id_e6b575-58 > .kt-row-column-wrap > div:not(.added-for-specificity){grid-column:initial;}}@media all and (max-width: 1024px){#kt-layout-id_e6b575-58 > .kt-row-column-wrap{grid-template-columns:repeat(3, minmax(0, 1fr));}}@media all and (max-width: 767px){#kt-layout-id_e6b575-58 > .kt-row-column-wrap > div:not(.added-for-specificity){grid-column:initial;}#kt-layout-id_e6b575-58 > .kt-row-column-wrap{grid-template-columns:minmax(0, 1fr);}}<\/style>\n<div class=\"wp-block-kadence-rowlayout alignnone\"><div id=\"kt-layout-id_e6b575-58\" class=\"kt-row-layout-inner  kt-layout-id_e6b575-58\"><div class=\"kt-row-column-wrap kt-has-3-columns kt-gutter-default kt-v-gutter-default kt-row-valign-top kt-row-layout-equal kt-tab-layout-inherit kt-m-colapse-left-to-right kt-mobile-layout-row kt-custom-first-width-15 kt-custom-second-width-75 kt-custom-third-width-10\"><style>.kadence-column_72899d-44 > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_72899d-44 > .kt-inside-inner-col,.kadence-column_72899d-44 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_72899d-44 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_72899d-44 > .kt-inside-inner-col{flex-direction:column;}.kadence-column_72899d-44 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_72899d-44 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_72899d-44{position:relative;}@media all and (max-width: 1024px){.kadence-column_72899d-44 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_72899d-44 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-1 kadence-column_72899d-44\"><div class=\"kt-inside-inner-col\">\n<p><\/p>\n<\/div><\/div>\n\n\n<style>.kadence-column_9a70fa-7e > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_9a70fa-7e > .kt-inside-inner-col,.kadence-column_9a70fa-7e > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_9a70fa-7e > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_9a70fa-7e > .kt-inside-inner-col{flex-direction:column;}.kadence-column_9a70fa-7e > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_9a70fa-7e > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_9a70fa-7e{position:relative;}@media all and (max-width: 1024px){.kadence-column_9a70fa-7e > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_9a70fa-7e > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-2 kadence-column_9a70fa-7e\"><div class=\"kt-inside-inner-col\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/11\/PCP_schema_opraveno.png\" alt=\"\" class=\"wp-image-6971\" width=\"539\" height=\"318\" srcset=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/11\/PCP_schema_opraveno.png 904w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/11\/PCP_schema_opraveno-300x177.png 300w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/11\/PCP_schema_opraveno-768x454.png 768w\" sizes=\"auto, (max-width: 539px) 100vw, 539px\" \/><\/figure><\/div>\n<\/div><\/div>\n\n\n<style>.kadence-column_1a6125-e3 > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_1a6125-e3 > .kt-inside-inner-col,.kadence-column_1a6125-e3 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_1a6125-e3 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_1a6125-e3 > .kt-inside-inner-col{flex-direction:column;}.kadence-column_1a6125-e3 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_1a6125-e3 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_1a6125-e3{position:relative;}@media all and (max-width: 1024px){.kadence-column_1a6125-e3 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_1a6125-e3 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-3 kadence-column_1a6125-e3\"><div class=\"kt-inside-inner-col\">\n<p><\/p>\n<\/div><\/div>\n<\/div><\/div><\/div>\n\n\n\n\n\n<h4><strong>3) Role of Wnt signaling in the Chronic Lymphocytic Leukemia (CLL)<\/strong><\/h4>\n<p>Our lab has shown recently that the&nbsp;<strong>PCP signaling<\/strong>&nbsp;pathway drives&nbsp;<strong>pathogenesis of Chronic Lymphocytic Leukemia (CLL)<\/strong>&nbsp;by controlling migration of CLL cells and their interaction with the microenvironment. Other labs have described similar role of the PCP pathway in other pathological situations such as metastatic spread.&nbsp;<strong>Targeting PCP signal transduction<\/strong>, in our hands mainly via&nbsp;<strong>innovative inhibitors of Casein kinase 1 (CK1)<\/strong>, thus represents a novel way of intervention for these largely incurable conditions. In this regard, we are trying to understand in detail the importance of PCP pathway for CLL. Namely, we aim to uncover its role in the&nbsp;<strong>CLL cell polarization and migration and in the communication of CLL cells with their environment<\/strong>. We actively explore the possibility to pharmacologically interfere with the PCP pathway and develop small compounds with this capacity.<\/p>\n\n\n<style>#kt-layout-id_65918a-55 > .kt-row-column-wrap{align-content:start;}:where(#kt-layout-id_65918a-55 > .kt-row-column-wrap) > .wp-block-kadence-column{justify-content:start;}#kt-layout-id_65918a-55 > .kt-row-column-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:var(--global-kb-gap-md, 2rem);padding-top:var( --global-kb-row-default-top, 25px );padding-bottom:var( --global-kb-row-default-bottom, 25px );padding-top:0px;grid-template-columns:minmax(0, calc(15% - ((var(--global-kb-gap-md, 2rem) * 2 )\/3)))minmax(0, calc(75% - ((var(--global-kb-gap-md, 2rem) * 2 )\/3)))minmax(0, calc(10% - ((var(--global-kb-gap-md, 2rem) * 2 )\/3)));}#kt-layout-id_65918a-55 > .kt-row-layout-overlay{opacity:0.30;}@media all and (max-width: 1024px){#kt-layout-id_65918a-55 > .kt-row-column-wrap > div:not(.added-for-specificity){grid-column:initial;}}@media all and (max-width: 1024px){#kt-layout-id_65918a-55 > .kt-row-column-wrap{grid-template-columns:repeat(3, minmax(0, 1fr));}}@media all and (max-width: 767px){#kt-layout-id_65918a-55 > .kt-row-column-wrap > div:not(.added-for-specificity){grid-column:initial;}#kt-layout-id_65918a-55 > .kt-row-column-wrap{grid-template-columns:minmax(0, 1fr);}}<\/style>\n<div class=\"wp-block-kadence-rowlayout alignnone\"><div id=\"kt-layout-id_65918a-55\" class=\"kt-row-layout-inner  kt-layout-id_65918a-55\"><div class=\"kt-row-column-wrap kt-has-3-columns kt-gutter-default kt-v-gutter-default kt-row-valign-top kt-row-layout-equal kt-tab-layout-inherit kt-m-colapse-left-to-right kt-mobile-layout-row kt-custom-first-width-15 kt-custom-second-width-75 kt-custom-third-width-10\"><style>.kadence-column_5ef596-91 > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_5ef596-91 > .kt-inside-inner-col,.kadence-column_5ef596-91 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_5ef596-91 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_5ef596-91 > .kt-inside-inner-col{flex-direction:column;}.kadence-column_5ef596-91 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_5ef596-91 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_5ef596-91{position:relative;}@media all and (max-width: 1024px){.kadence-column_5ef596-91 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_5ef596-91 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-1 kadence-column_5ef596-91\"><div class=\"kt-inside-inner-col\">\n<p><\/p>\n<\/div><\/div>\n\n\n<style>.kadence-column_81cfda-40 > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_81cfda-40 > .kt-inside-inner-col,.kadence-column_81cfda-40 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_81cfda-40 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_81cfda-40 > .kt-inside-inner-col{flex-direction:column;}.kadence-column_81cfda-40 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_81cfda-40 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_81cfda-40{position:relative;}@media all and (max-width: 1024px){.kadence-column_81cfda-40 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_81cfda-40 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-2 kadence-column_81cfda-40\"><div class=\"kt-inside-inner-col\">\n<div class=\"wp-block-image\"><figure class=\"aligncenter is-resized\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Research_3.jpg\" alt=\"\" class=\"wp-image-4631\" width=\"559\" height=\"440\" srcset=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Research_3.jpg 982w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Research_3-300x236.jpg 300w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2020\/04\/Research_3-768x605.jpg 768w\" sizes=\"auto, (max-width: 559px) 100vw, 559px\" \/><\/figure><\/div>\n<\/div><\/div>\n\n\n<style>.kadence-column_a48569-86 > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_a48569-86 > .kt-inside-inner-col,.kadence-column_a48569-86 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_a48569-86 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_a48569-86 > .kt-inside-inner-col{flex-direction:column;}.kadence-column_a48569-86 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_a48569-86 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_a48569-86{position:relative;}@media all and (max-width: 1024px){.kadence-column_a48569-86 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_a48569-86 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-3 kadence-column_a48569-86\"><div class=\"kt-inside-inner-col\"><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n\n<h4><strong>4) Extracellular vesicles in ovarian cancer and the potential of malignant ascites for ovarian cancer research<br \/><\/strong><\/h4>\n<p>Ovarian cancer (OC) ranks among the deadliest cancers in women. Lack of symptoms, rapid metastases and common chemoresistance contribute to unfortunate fate of majority of OC patients, especially those having high-grade serous carcinoma of the ovary, fallopian tube and peritoneum (HGSC), the most common and most aggressive type of OC. Many HGSC patients have excess fluid in the peritoneum called ascites. Ascites is basically a tumor microenvironment (TME) containing various cells, proteins and extracellular vesicles (EVs).<\/p>\n<p><br \/>EVs play an important role in cancerogenesis and hold great promise as disease biomarkers, yet their small size and polydispersity bring various challenges to their isolation and characterization, including method-dependent enrichment of different EV subtypes as well as contaminants.<\/p>\n<p><br \/>Our research projects focus on method development in the field, comparison of different isolation methods and especially on proteomic analyses of ascitic EVs and how these can help in unraveling the underlying molecular principles of disease progression and how can ascites serve as a new source of diagnostic\/screening\/prognostic EV-based biomarkers of HGSC and clinically relevant disease models.<\/p>\n<p>We are proud members of the <a href=\"https:\/\/www.isev.org\/\">International Society for Extracellular Vesicles (ISEV)<\/a> and co-founders of <a href=\"https:\/\/webcentrum.muni.cz\/czesev\/en\">Czech Society for Extracellular Vesicles (CzeSEV)<\/a>.<\/p>\n\n\n<style>#kt-layout-id_e9b6fe-dc > .kt-row-column-wrap{align-content:start;}:where(#kt-layout-id_e9b6fe-dc > .kt-row-column-wrap) > .wp-block-kadence-column{justify-content:start;}#kt-layout-id_e9b6fe-dc > .kt-row-column-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:var(--global-kb-gap-md, 2rem);padding-top:var( --global-kb-row-default-top, 25px );padding-bottom:var( --global-kb-row-default-bottom, 25px );padding-top:var(--global-kb-spacing-sm, 1.5rem);padding-bottom:var(--global-kb-spacing-sm, 1.5rem);grid-template-columns:minmax(0, calc(10% - ((var(--global-kb-gap-md, 2rem) * 2 )\/3)))minmax(0, calc(80% - ((var(--global-kb-gap-md, 2rem) * 2 )\/3)))minmax(0, calc(10% - ((var(--global-kb-gap-md, 2rem) * 2 )\/3)));}#kt-layout-id_e9b6fe-dc > .kt-row-layout-overlay{opacity:0.30;}@media all and (max-width: 1024px){#kt-layout-id_e9b6fe-dc > .kt-row-column-wrap > div:not(.added-for-specificity){grid-column:initial;}}@media all and (max-width: 1024px){#kt-layout-id_e9b6fe-dc > .kt-row-column-wrap{grid-template-columns:repeat(3, minmax(0, 1fr));}}@media all and (max-width: 767px){#kt-layout-id_e9b6fe-dc > .kt-row-column-wrap > div:not(.added-for-specificity){grid-column:initial;}#kt-layout-id_e9b6fe-dc > .kt-row-column-wrap{grid-template-columns:minmax(0, 1fr);}}<\/style>\n<div class=\"wp-block-kadence-rowlayout alignnone\"><div id=\"kt-layout-id_e9b6fe-dc\" class=\"kt-row-layout-inner  kt-layout-id_e9b6fe-dc\"><div class=\"kt-row-column-wrap kt-has-3-columns kt-gutter-default kt-v-gutter-default kt-row-valign-top kt-row-layout-equal kt-tab-layout-inherit kt-m-colapse-left-to-right kt-mobile-layout-row kt-custom-first-width-10 kt-custom-second-width-80 kt-custom-third-width-10\"><style>.kadence-column_46c9ce-d3 > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_46c9ce-d3 > .kt-inside-inner-col,.kadence-column_46c9ce-d3 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_46c9ce-d3 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_46c9ce-d3 > .kt-inside-inner-col{flex-direction:column;}.kadence-column_46c9ce-d3 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_46c9ce-d3 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_46c9ce-d3{position:relative;}@media all and (max-width: 1024px){.kadence-column_46c9ce-d3 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_46c9ce-d3 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-1 kadence-column_46c9ce-d3\"><div class=\"kt-inside-inner-col\">\n<p><\/p>\n<\/div><\/div>\n\n\n<style>.kadence-column_1109cd-14 > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_1109cd-14 > .kt-inside-inner-col,.kadence-column_1109cd-14 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_1109cd-14 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_1109cd-14 > .kt-inside-inner-col{flex-direction:column;}.kadence-column_1109cd-14 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_1109cd-14 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_1109cd-14{position:relative;}@media all and (max-width: 1024px){.kadence-column_1109cd-14 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_1109cd-14 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-2 kadence-column_1109cd-14\"><div class=\"kt-inside-inner-col\">\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"605\" height=\"168\" src=\"https:\/\/www.sci.muni.cz\/ofiz\/wp-content\/uploads\/2023\/09\/czesevjpg.jpg\" alt=\"\" class=\"wp-image-10842\" srcset=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2023\/09\/czesevjpg.jpg 605w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2023\/09\/czesevjpg-300x83.jpg 300w, https:\/\/ueb1.sci.muni.cz\/ofiz\/wp-content\/uploads\/2023\/09\/czesevjpg-370x103.jpg 370w\" sizes=\"auto, (max-width: 605px) 100vw, 605px\" \/><\/figure>\n<\/div><\/div>\n\n\n<style>.kadence-column_35a5fd-83 > .kt-inside-inner-col{border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;}.kadence-column_35a5fd-83 > .kt-inside-inner-col,.kadence-column_35a5fd-83 > .kt-inside-inner-col:before{border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;}.kadence-column_35a5fd-83 > .kt-inside-inner-col{column-gap:var(--global-kb-gap-sm, 1rem);}.kadence-column_35a5fd-83 > .kt-inside-inner-col{flex-direction:column;}.kadence-column_35a5fd-83 > .kt-inside-inner-col > .aligncenter{width:100%;}.kadence-column_35a5fd-83 > .kt-inside-inner-col:before{opacity:0.3;}.kadence-column_35a5fd-83{position:relative;}@media all and (max-width: 1024px){.kadence-column_35a5fd-83 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}@media all and (max-width: 767px){.kadence-column_35a5fd-83 > .kt-inside-inner-col{flex-direction:column;justify-content:center;}}<\/style>\n<div class=\"wp-block-kadence-column inner-column-3 kadence-column_35a5fd-83\"><div class=\"kt-inside-inner-col\"><\/div><\/div>\n<\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>V\u00edt\u011bzslav Bryja &#8211; Laboratory of Cellular Communication Introduction Morphogenetic proteins from the&nbsp;Wnt family&nbsp;are important regulators of&nbsp;embryonal development. They have also huge impact on&nbsp;homeostasis regulation&nbsp;of the adult organism. Deregulation of Wnt pathway is associated with&nbsp;cancer and many other diseases. Although the &hellip; <a href=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/bryja\/\">Read More<\/a><\/p>\n","protected":false},"author":3,"featured_media":1081,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-feature.php","meta":{"kt_blocks_editor_width":"","_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"footnotes":""},"categories":[236],"tags":[],"class_list":["post-668","page","type-page","status-publish","has-post-thumbnail","hentry","category-research-teams"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.1.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>V\u00edt\u011bzslav Bryja - Department of Animal Physiology and Immunology<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/ueb1.sci.muni.cz\/ofiz\/en\/bryja\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"V\u00edt\u011bzslav Bryja - Department of Animal Physiology and Immunology\" \/>\n<meta property=\"og:description\" content=\"V\u00edt\u011bzslav Bryja &#8211; Laboratory of Cellular Communication Introduction Morphogenetic proteins from the&nbsp;Wnt family&nbsp;are important regulators of&nbsp;embryonal development. They have also huge impact on&nbsp;homeostasis regulation&nbsp;of the adult organism. Deregulation of Wnt pathway is associated with&nbsp;cancer and many other diseases. 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